Native PAGE methods/recipes

Kevin Brady rcxkb at unix.ccc.nottingham.ac.uk
Wed Jul 11 05:50:05 EST 2001


Hi,  I'm looking for a good protocol for doing Native PAGE on a protein
I've purified. The theoretical pI (as calculated at Expasy ProtParam) is
7.03. Only ever done SDS-PAGE, so any help would be appreciated on gel
compositions and buffers.
Cheers
Kevin

--
Dr Kevin Brady
Organic Chemistry Section
School of Chemistry
University of Nottingham
Nottingham
UK
NG7 2RD
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Email : Kevin.Brady at nottingham.ac.uk or
        kevin at basil.chem.nott.ac.uk
URL   : http://www.nottingham.ac.uk/~pcxsc/thomas
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