stored RNA - is it any good
C.W.Herbert at liverpool.ac.uk
Thu Jul 12 08:11:24 EST 2001
There are 2 ways to check the RNA quality. Firstly you should determine
the purity of the RNA using the A260/A280 ratio. This should be around 1.9
for good quality RNA. The second way is to run approximately 1 microgram of
the RNA (determined from the A260 value) on a 1% agarose gel for 1 hour at
60V and look for the presence 28S and 18S rRNA. These should be show as
distinct bands on the gel with the 28S band at roughly 2-3 times the
intensity of the 18S rRNA band. If you can't see these bands then the RNA
is degraded and shouldn't be used for RT-PCR or Microarrays as the quality
of data obtained from these is dependant on having high quality template RNA.
I hope this is of some use. If you need any further advice don't hesitate
to contact me.
More information about the Methods