C.W.Herbert at liverpool.ac.uk
Thu Jul 12 08:19:31 EST 2001
I am currently carrying out some Real-Time PCR experiments (using a
Roche Lightcycler) on tumour and non-tumour tissue samples. This is a very
sensitive technique and I am currently having trouble identifying a reliable
housekeeping genes to use as an internal standards for these experiments. I
am reluctant to use GAPDH because it has been suggested recently that levels
of GAPDH can vary in times of cellular stress.
Can anybody suggest other housekeeping genes that I can use?
Many thanks for your help.
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