I am afraid I got wrong Primer from company

Sergio sergioal at bbm1.ucm.es
Thu Jul 12 12:02:38 EST 2001



yoel and adaia shiboleth wrote:

> Is it the same base every time? if not, your gene product is toxic and your
> plasmid has a leaky promoter and only mutated clones survive. I'm experiencing
> this problem at the moment myself. Can probably be dealt with with introns or
> a change of vector/ bacterial strain. We also blaimed the primer at first...
> Yoel

Yes... same happened in our lab. Just switching to a tightly controled
expression vector solved the problem.

Sergio




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