blotting of a IPG electrophoresis

Stefi~ xxxgifalone at tin.it
Thu Jul 12 16:28:20 EST 2001


Hello. I have a problem. I cannot find the protocol to do a blotting of a
IPG electrophoresis. I have to separate two proteins with almost the same
molecular weight but different isoeletric point (7.64 and 8.22). I think
I'll use a 5.5-8.5 gradient gel but I have to know the conditions for
blotting: buffer, voltage, amp, time, etc.
Can anyone help me? Thanx a lot.
Stefano
gifalone at tin.it

http://web.tiscalinet.it/gifalone
ICQ 110419911





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