Ethidium bromide

L. Rzepiel lrzepiel at uoguelph.ca
Thu Jul 12 18:47:30 EST 2001


You do not need to run your gel in a fumehood.  No one ever does it.
You also do not need to treat the running buffer as waste, it can go
down the drain.  As far as using EtBr, you can just put it into your
agarose and swirl, right before you pour your gel.

LR

On Thu, 5 Jul 2001 13:52:36 +0200, "salanti" <ali at image.dk> wrote:

>Hi.
>In our lab we have so far stained our DNA agarosegels with ethidium bromide
>after electrophoreses. The water we have washed the gels in after ethidium
>bromide staining is collected and run through a column that should bind
>ethidiumbromide. In order to minimize our use of Ethidium bromide we have
>talked about mixing the samples with loading dye + ethidumbromide.
>My questions are then: Is it necessary to run this gel in a fume hood? And
>what about the running buffer, is it necessary to collect this and treat it
>as chemical waste????
>I have tried to obtain this information from the right authorities in my
>country, but have failed! Does anyone have a suggestion??
>
>
>




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