jakku at mrna.tn.nic.in
Fri Jul 13 00:27:28 EST 2001
you don't need to do all that. We never much bother about the used water,
let alone running it through binding columns. Just dump it into the nearest
drain. If you need to deactivate Ethbr, just incinerate the solution.. or
boil it. It is very sensitive to heat.
----- Original Message -----
From: "L. Rzepiel" <lrzepiel at uoguelph.ca>
To: <methods at hgmp.mrc.ac.uk>
Sent: Friday, July 13, 2001 5:17 AM
Subject: Re: Ethidium bromide
> You do not need to run your gel in a fumehood. No one ever does it.
> You also do not need to treat the running buffer as waste, it can go
> down the drain. As far as using EtBr, you can just put it into your
> agarose and swirl, right before you pour your gel.
> On Thu, 5 Jul 2001 13:52:36 +0200, "salanti" <ali at image.dk> wrote:
> >In our lab we have so far stained our DNA agarosegels with ethidium
> >after electrophoreses. The water we have washed the gels in after
> >bromide staining is collected and run through a column that should bind
> >ethidiumbromide. In order to minimize our use of Ethidium bromide we have
> >talked about mixing the samples with loading dye + ethidumbromide.
> >My questions are then: Is it necessary to run this gel in a fume hood?
> >what about the running buffer, is it necessary to collect this and treat
> >as chemical waste????
> >I have tried to obtain this information from the right authorities in my
> >country, but have failed! Does anyone have a suggestion??
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