Soaking in nucleic acids into a PAGE gel
tyr-2 at bones.biochem.ualberta.ca
Fri Jul 13 10:04:18 EST 2001
> Given that the polymerase is a DNA binding protein, that is not too
> unexpected. I wonder if he can blot the protein out onto nitrocellulose
> and incubate the nitrocellulose with the polynucleotide in solution.
> It's a bit like Boehringer's as was patent for assaying recombinant
> pols. By blotting E.coli colonies to nitrocellulose, heat lysing the
> colonies on the filter and assaying using radiolabel. The template was
> that present in the lysed colony. I tried their method a few years ago
> after it was published in Gene and it worked great.
Thanks for the suggestion about the blot-immobilized enzyme assay. I'll pass
the info on to my colleague.
More information about the Methods