lucv at mail.med.upenn.edu
Fri Jul 13 13:23:47 EST 2001
"L. Rzepiel" wrote:
> You do not need to run your gel in a fumehood. No one ever does it.
> You also do not need to treat the running buffer as waste, it can go
> down the drain. As far as using EtBr, you can just put it into your
> agarose and swirl, right before you pour your gel.
The problem with this strategy is that your EtBr will migrate in the
opposite direction of your DNA during electrophoresis. This will make
that the band with a lower molecular weigth will be less visible.
Soaking the gel in a 1ug/ml EtBr solution for 10 min eradicates that
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