sensitivity of Western Blottings

Dima Klenchin klenchin at REMOVE_TO_REPLY.facstaff.wisc.edu
Sat Jul 14 11:11:59 EST 2001


"Stefi~" <xxxgifalone at tin.it> wrote:
:Hello, does anyone know the sensitivity of a Western blotting with these 2
:methods of revelations:
:ECL;
:H2O2, chloro-naphtol and diaminobenzidine

Thereis no such thing as absolute sensitivity on Western. Most of 
all other things, it depends on specific antigen and antibody, and 
method of detection (three-layer systems being more sensitive than 
stadard two-layer techniques). ECL is certainly a lot more sensitive 
than colorimetric methods. In any case, the limit of sensitivity 
will usually be found in the range of 10 ng-10 pg. 

        - Dima




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