sensitivity of Western Blottings
xxxgifalone at tin.it
Sat Jul 14 12:49:45 EST 2001
Dima Klenchin <klenchin at REMOVE_TO_REPLY.facstaff.wisc.edu> wrote in message
9ipr0f$21c_002 at doit.wisc.edu...
> Thereis no such thing as absolute sensitivity on Western. Most of
> all other things, it depends on specific antigen and antibody, and
> method of detection (three-layer systems being more sensitive than
> stadard two-layer techniques). ECL is certainly a lot more sensitive
> than colorimetric methods. In any case, the limit of sensitivity
> will usually be found in the range of 10 ng-10 pg.
thanx a lot.
gifalone at tin.it
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