plating from chlor onto amp

R. Jayakumar jakku at mrna.tn.nic.in
Mon Jul 16 04:51:55 EST 2001


Isn't is possible to plate the transformants on Amp, Chlor plates instead of
only on Ap or Cm plates.
   best of luck
jayakumar

----- Original Message -----
From: "Frederik Wirtz-Peitz" <fw218 at cam.ac.uk>
To: <methods at hgmp.mrc.ac.uk>
Sent: Monday, July 16, 2001 4:22 AM
Subject: plating from chlor onto amp


> Dear all,
>
> I am trying to clone an insert which contains a chloramphenicol marker
into
> a vector which features an ampicillin marker. I plated my ligation onto
> chlor plates, then streaked out individual colonies on an amp plate, from
> which I picked colonies for mini-prep. I am now worried that, since chlor
> isn't lethal but only inhibits growth, there may be significant background
> of re-circularized vector on the amp plates (defeating the purpose of
> plating on chlor in the first place). Does anyone know if this is a valid
> concern? (I only just set up the mini-preps so I don't know the result
yet.)
>
> Thanks.
>
>
>

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