Ethidium bromide

J. Rinehart jrineharNOSPAM at snake.eou.edu
Mon Jul 16 09:32:08 EST 2001



Luk Vandenberghe wrote:

> "L. Rzepiel" wrote:
> >
> > You do not need to run your gel in a fumehood.  No one ever does it.
> > You also do not need to treat the running buffer as waste, it can go
> > down the drain.  As far as using EtBr, you can just put it into your
> > agarose and swirl, right before you pour your gel.
> >
> > LR
>
> The problem with this strategy is that your EtBr will migrate in the
> opposite direction of your DNA during electrophoresis. This will make
> that the band with a lower molecular weigth will be less visible.
> Soaking the gel in a 1ug/ml EtBr solution for 10 min eradicates that
> problem.
>
> LV

We find also that adding EtBr directly to the running buffer as well as
the gel eliminates this problem. No soaking, destaining, or other annoying
step is necessary. Just add EtBr to the same concentration as in your gel.
Background is very minimal in our experience.

J. Rinehart




More information about the Methods mailing list