COS-7 Cell Electroporation

[Louis Hom]

I'm new to electroporating mammalian cells and I'd appreciate any tips on
what parameters I should pay attention to most for transient expression
(e.g., preincubation, electroporation medium, recovery medium).  I 
apologize for such a broad request, but I read a collection of user 
surveys on Bio-Rad's site and they all seem to say different things.  
Thanks.
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______________________________________________________________________________
Lou Hom >K'93			     
lhom at ocf.berkeley.edu		
http://www.ocf.berkeley.edu/~lhom/