bubbles and agar plates

Sergio sergioal at bbm1.ucm.es
Tue Jul 17 15:49:08 EST 2001

I suggest you to not store the plates at 4ºC immediately pouring them. We use to
let the plates on the bench at least 24 h, and put them into the refrigerator
afterwards. This reduces a lot the bubble formation inside the agar, maybe also
beneath it.


John Rebers wrote:

> Occasionally I have problems with agar plates used for growing bacteria
> with small bubbles (2-3 mm in diameter) appearing between the plate and the
> agar medium. These are not on the surface of the plate or within the agar
> itself, but are air bubbles at the bottom of the plate between the medium
> and the agar. The bubbles are invisible at room temperature, but expand
> significantly when the plates are incubated at 37C. They are sometimes
> abundant enough to make it more difficult to distinguish colonies on the
> plate surface. Since they only show up some of the time, I suspect this has
> something to do with the way the plates are poured - the temperature of the
> medium, perhaps. Has anyone else seen these and figure out how to avoid them?
> Thanks,
> John E. Rebers
> Department of Biology
> Northern Michigan University
> 1401 Presque Isle Avenue
> Marquette, MI  49855
> jrebers at nmu.edu
> 906-227-1585; 906-227-1063 (FAX)
> ---

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