Ethidium bromide (-> Ames test)
roland.hubner at ua.ac.be
Tue Jul 17 16:17:37 EST 2001
In article <170720012125260261%dmicklem at cmgm.nospam.invalid>, David
Micklem <dmicklem at cmgm.nospam.invalid> wrote:
> In article <3b544827$1 at news.uia.ac.be>, Roland Hubner
> <roland.hubner at ua.ac.be> wrote:
> > If sodium azide has been so well studied, I suspect that for EtBr
> >(highly mutagenic to procaryotes! -- bacteria in Ames test etc.) some
> >published work should be around, right?
> Ah, the hysteria surrounding ethidium bromide! How many of you out
> there know grad students who have given themselves a 'sunburn' on the
> shortwave UV gel box (UV exposure = well established risk factor for
> melanoma) while being ultra-paranoid about the ethidium bromide
> (apparently an acute irritant, but evidence for other harmful effects
> seems elusive).
> Does anyone have a reference for the Ames test result for ethidium
> bromide? The MSDS lists Ethidium bromide as:
> carcinogenicity - NTP: No
> carcinogenicity - IARC: No
> carcinogenicity - OSHA: No
> but doesn't seem to mention the Ames test.
> Now I realise that carcinogenicity is not equivalent to mutagenicity
> but still...
> Also, I believe EthBr binds rather well to proteins, so it may be
> unlikely to make it through the skin, let alone into the nucleus.
> Someone else mentioned they'd heard of its use as an anti-sleeping
> sickness therapeutic drug (for cattle). I think that is documented in
> the Merck Index but I don't have access to a copy at the moment. It
> would be interesting to know whether anyone ever looked for/found
> adverse effects on the cattle.
check out refs in:
Singer VL, Lawlor TE, Yue S. "Comparison of SYBR Green I nucleic acid
gel stain mutagenicity and ethidium bromide mutagenicity in the
Salmonella/mammalian microsome reverse mutation assay (Ames test)"
Mutat Res 1999 Feb 2;439(1):37-47
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