Pichia secretion proteins

W. Stuart Annan wsa at st-andrews.ac.uk
Wed Jul 18 04:55:47 EST 2001


Hi,

Does anyone know what proteins are naturally secreted by Pichia into the
culture media? I've read somewhere it's about 4-5 proteins but, I can't
find any mention of what they are.

The reason behind my request is that I've been having trouble getting my
protein of interest to bind to Nickle column and I'm now going to try ion
exchange chromatography and it would be useful to know what proteins may
also bind to the column. 

Stuart Annan

Structural Biology
University of St. Andrews

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