Coomassie anti-bands

Richard P. Grant rpg14 at yahoo.co.uk.invalid
Wed Jul 18 07:35:39 EST 2001


In article 
<Pine.SGI.4.33.0107181314480.2513510-100000 at mole.bio.cam.ac.uk>,
 Michael Witty <mw132 at mole.bio.cam.ac.uk> wrote:

> protein gels:  I have a gel, stained with Coomassie blue which has a very
> light blue background and darker blue bands BUT also some things which
> look like protein bands (about 20kDa) but are less blue than the background.
> Whatever it is seems to be repelling the Coomassie blue!  Mike.
> 

Hi Mike,

That usually means your Coomassie is going off - i.e. the methanol has 
evaporated.  Time to make some fresh :)

Tip - I make up two litres at a time and store it in a cupboard in the 
coldroom.  I use about 100 ml as my working stock, good for a dozen 
gels, probably many more.

r

-- 
Richard P. Grant  0x5F9559B1                         MRC Lab of Mol Biol
rpg 'at' mrc-lmb.cam.ac.uk   http://www2.mrc-lmb.cam.ac.uk/personal/rpg/
                   -- 'Immunologically flaccid' --




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