Richard P. Grant
rpg14 at yahoo.co.uk.invalid
Wed Jul 18 07:35:39 EST 2001
<Pine.SGI.4.33.0107181314480.2513510-100000 at mole.bio.cam.ac.uk>,
Michael Witty <mw132 at mole.bio.cam.ac.uk> wrote:
> protein gels: I have a gel, stained with Coomassie blue which has a very
> light blue background and darker blue bands BUT also some things which
> look like protein bands (about 20kDa) but are less blue than the background.
> Whatever it is seems to be repelling the Coomassie blue! Mike.
That usually means your Coomassie is going off - i.e. the methanol has
evaporated. Time to make some fresh :)
Tip - I make up two litres at a time and store it in a cupboard in the
coldroom. I use about 100 ml as my working stock, good for a dozen
gels, probably many more.
Richard P. Grant 0x5F9559B1 MRC Lab of Mol Biol
rpg 'at' mrc-lmb.cam.ac.uk http://www2.mrc-lmb.cam.ac.uk/personal/rpg/
-- 'Immunologically flaccid' --
More information about the Methods