clean hamilton 0.2mm syringae

[Michael Witty]

Dear Felipe,
           do you have SDS in your buffer?  In which case precipitation
may be taking place if the rinsing buffer is too cold: use warm buffer?
(using warm water or ethanol after the event may be no good because a
tight plug forms).  Mike.

On Fri, 20 Jul 2001, felipe wettstein wrote:

> wo load our sequencing gels with a 8 channel 0.2mm gas tight hamilton
> syringae. but quite often a syringae blocks, and cannot be cleaned any
> more.
> we rinse the syringaes bevor and after gelloading with dd water and the
> full volume. and we tried to clean the blocked ones very carefully with
> water, with warm water, with 96%EtOH.. whatever. nothing helped. can
> anybody give me a hint?
> thank you.
>
> felipe
>