clean hamilton 0.2mm syringae
felipe.wettstein at ioez.tu-freiberg.de
Fri Jul 20 06:57:06 EST 2001
> do you have SDS in your buffer? In which case precipitation
no, as far as i know there is no sds in the loading buffer. the buffer
basic fuchsin, edta, bromophenolblue and formamide. and the samples
loaded, contian the buffers from the autosequencing pcr.
> may be taking place if the rinsing buffer is too cold: use warm buffer?
we rinse with double destilled water, i assume it is at about room
> (using warm water or ethanol after the event may be no good because a
> tight plug forms). Mike.
that is an interesting point. do you think that the proteins (especially
taq polymerase) remainings could denaturate and block the syringae?
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