clean hamilton 0.2mm syringae

Michael Witty mw132 at mole.bio.cam.ac.uk
Fri Jul 20 07:24:19 EST 2001


> > may be taking place if the rinsing buffer is too cold: use warm buffer?
> we rinse with double destilled water, i assume it is at about room
> temperature.

...have you tried rinsing in running buffer?  Ie just dip the needle in
the gel buffer tank and rinse - presumably the sample buffer does not
precipitate in running buffer otherwise you would notice an artefact
during a run

> > (using warm water or ethanol after the event may be no good because a
> > tight plug forms).  Mike.
> that is an interesting point. do you think that the proteins (especially
> taq polymerase) remainings could denaturate and block the syringae?

No.  There wouldn't be enough would there?  Regards, Mike.




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