clean hamilton 0.2mm syringae
felipe.wettstein at ioez.tu-freiberg.de
Fri Jul 20 12:18:01 EST 2001
> > > may be taking place if the rinsing buffer is too cold: use warm buffer?
> > we rinse with double destilled water, i assume it is at about room
> > temperature.
> ...have you tried rinsing in running buffer? Ie just dip the needle in
> the gel buffer tank and rinse - presumably the sample buffer does not
> precipitate in running buffer otherwise you would notice an artefact
> during a run
and you have no problems with blocked syringaes? to rinse in running
buffer is the first thought, i usually did before, but then suddenly i
changed to water, .. hm... i will try.
More information about the Methods