clean hamilton 0.2mm syringae

[David L Haviland]

Hi:

For cleaning Hamilton syringes I always used a combination of 4 
solutions.  1) DDH20, 2) 2% SDS, 3) 70% EtOH, and 4) Acetone.    
Go from 1 to 2, back to 1, then to 3, and finally 4, pulling about 10 
mls through each time.  Use a 200ul pipette tip that is cut off that 
leads to an aspirator and stick the narrow end in the back of the 
Hamilton.  Clean the plunger by dipping in the four solutions and 
wiping down each time.

As a result, I've never lost a Hamilton this way and have been able 
to "re-claim" a few from the destiny of the trash can!!

Hope this helps,
David

============ 
David L. Haviland, Ph.D., Asst. Prof. Immunology 
University of Texas - Houston, H.S.C. 
Institute of Molecular Medicine, R907 
2121 W. Holcombe Blvd.,  Houston, TX  77030 
713.500.2413-Voice//713.500.2424-FAX 
----------------- 
If everything seems to be going so well, you have obviously 
overlooked something. 
============

----- Original Message -----
From: felipe wettstein <felipe.wettstein at ioez.tu-freiberg.de>
Date: Friday, July 20, 2001 10:18 am
Subject: Re: clean hamilton 0.2mm syringae

> 
> > > > may be taking place if the rinsing buffer is too cold: use 
> warm buffer?
> > > we rinse with double destilled water, i assume it is at about room
> > > temperature.
> > 
> > ...have you tried rinsing in running buffer?  Ie just dip the 
> needle in
> > the gel buffer tank and rinse - presumably the sample buffer 
> does not
> > precipitate in running buffer otherwise you would notice an artefact
> > during a run
> and you have no problems with blocked syringaes? to rinse in running
> buffer is the first thought, i usually did before, but then 
> suddenly i
> changed to water, .. hm... i will try.
> 
> thank you
> 
> felipe
> 
> 

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