Coomassie anti-bands

Bryan Maloney bjm10 at cornell.edu
Sat Jul 21 00:15:08 EST 2001


Michael Witty <mw132 at mole.bio.cam.ac.uk> wrote in
news:Pine.SGI.4.33.0107181314480.2513510-100000 at mole.bio.cam.ac.uk: 

> Dear Everyone,
>              have you ever had this artefact in Coomassie blue stained
> protein gels:  I have a gel, stained with Coomassie blue which has a
> very light blue background and darker blue bands BUT also some things
> which look like protein bands (about 20kDa) but are less blue than the
> background. Whatever it is seems to be repelling the Coomassie blue! 


Yes, only when I was doing something like expressing a phage coat protein in 
the phage's native host.  In a nutshell, There was so much of the protein 
that it was excluding the dye.

-- 
"Cape Cod Salsa--somehow that's just not right."




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