gottfried.dasen at ilw.agrl.ethz.ch
Mon Jul 23 11:54:13 EST 2001
I have two questions concerning the TaqMan PCR technique:
I want to detect the "total bacterial DNA" load of various samples
(food, ....). (in order to compare it with the incidence of other
Does anybody have a suggestion for a promising target gene (16S rDNA is
not a single copy gene, so its of no real use to me).
Ideally such a gene should be ubiquitous (well, I'll settle for: present
in most bugs), single copy, enough DNA-seqs published, ..
Do you have any suggestions ?
Since I'm going to quantitate DNA, I also must use a quantitative DNA
So far nobody seems to have payed attention to these fact or at least I
couldn't find any work on it.
Does anyone have experience with this ?
Thanks in advance !
Dr. Gottfried H. Dasen
ETH, Laboratory of Food Microbiology
CH-8092 Zurich, Switzerland
phone: +41 1 632 53 78
fax: +41 1 632 12 66
dasen at ilw.agrl.ethz.ch
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