sodium tetraborate as transferbuffer

[Frank O. Fackelmayer]

Borax wrote:
> 
> hello everybody!
> 
> i want to use sodium tetraborate as transferbuffer for sds-free western
> blotting. but i don't know which molarity i've to use and if this buffer is
> suitable for semidry and tank blotting.
> 


i haven´t tried it, but I´d guess that at least in tank blotting
standard TBE buffer (as used for agarose gels) should do the job.
Possibly it is a good idea to put membranes on both sides of the gel, as
your protein might run in the opposite direction under non-SDS conditions.

Frank