sodium tetraborate as transferbuffer

R. John Lye rjl6n at virginia.edu
Tue Jul 24 06:00:41 EST 2001


"Frank O. Fackelmayer" wrote:

> Borax wrote:
> >
> > hello everybody!
> >
> > i want to use sodium tetraborate as transferbuffer for sds-free western
> > blotting. but i don't know which molarity i've to use and if this buffer is
> > suitable for semidry and tank blotting.

Why not use 10 mM CAPs (pH 11.0) plus 10% methanol?  This
is the buffer that I've used when doing SDS-free transfers for protein
sequencing?

John





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