GST Contamination of GST-fusion proteins

Eric Eric.Merithew at umassmed.edu
Tue Jul 24 10:44:12 EST 2001


Does anyone have suggestions on a method of purifying a gst-fusion protein
from contaminating gst protein without cleaving the fusion protein.  Due to
gst dimerization, many of the column purification methods I have tried (ion
exchange, gel filtration) have been unsuccessful.

Thanks,
Eric 


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