Ethidium Bromide Fluorescence

Sergio sergioal at bbm1.ucm.es
Wed Jul 25 11:53:31 EST 2001


AFAIK ethidium bromide also fluoresces when not binded to DNA....

Carmelo wrote:

> In article (Dans l'article)
> <60805a5e.0107250646.1292beb7 at posting.google.com>, Bill Atkins
> <billatkins at bigfoot.com> wrote (écrivait) :
>
> > Why does ethidium bromide only fluoresce in the prescense of DNA?
> >
> > Thanks,
> > Bill
>
> Hello Bill,
>
> Something to do with changes in the electric dipole transition moment
> (movement of charge density from the ground to the excited state) of
> EtBr once stacked between the bases?
>
> Carmelo




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