mw132 at mole.bio.cam.ac.uk
Wed Jul 25 12:45:28 EST 2001
. . . why are you using A rhizogenes? Mike.
> I have been working with A. tumefaciens using the PZP vectors. I now want
> to switch to using A.rhizogenes, but don't have a vector that is specific
> for this strain.
> These are my questions:
> 1. Can I use the same PZP vectors ? Are the LB/RB sequences quite a bit
> different between species?
> 2. Would it just be a manner of replacing the LB and RB sequences with
> rhizogene sequences, or will I have to replace the agrobacterium
> in my plasmid that are used for replication and maintance of the vector
> 3. What alterations are made to Agrobacterium strains to make them
> in the lab for transformation? I notice that some scientists will even
> natural isolates, while other strains have had some modifications made.
> Thank you very much.
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