Agrobacterium vectors

Michael Witty mw132 at mole.bio.cam.ac.uk
Wed Jul 25 12:45:28 EST 2001


. . . why are you using A rhizogenes?  Mike.

>
> Hi,
>
> I have been working with A. tumefaciens using the PZP vectors. I now want
> to switch to using A.rhizogenes, but don't have a vector that is specific
> for this strain.
> These are my questions:
>
> 1.  Can I use the same PZP vectors ? Are the LB/RB sequences quite a bit
> different between species?
> 2.  Would it just be a  manner of replacing the LB and RB sequences with
> A.
> rhizogene sequences, or will I have to replace the agrobacterium
> sequences
> in my plasmid that are used for replication and maintance of the vector
> in
> Agrobacterium?
> 3.  What alterations are made to Agrobacterium strains to make them
> useful
> in the lab for transformation?  I notice that some scientists will even
> use
> natural isolates, while other strains have had some modifications made.
>
> Thank you very much.
>
> Sagy
>
>
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