blotting of a IPG electrophoresis

news.ind.net jclack at iupui.edu
Thu Jul 26 10:46:19 EST 2001


Try this (Bio-Rad instructions):

http://www.bio-rad.com/cmc_upload/0/000/013/280/M1703930E.pdf

"Stefi~" <xxxgifalone at tin.it> wrote in message
news:UDo37.3000$Pv.105168 at news.infostrada.it...
> Hello. I have a problem. I cannot find the protocol to do a blotting of a
> IPG electrophoresis. I have to separate two proteins with almost the same
> molecular weight but different isoeletric point (7.64 and 8.22). I think
> I'll use a 5.5-8.5 gradient gel but I have to know the conditions for
> blotting: buffer, voltage, amp, time, etc.
> Can anyone help me? Thanx a lot.
> Stefano
> gifalone at tin.it
>
> http://web.tiscalinet.it/gifalone
> ICQ 110419911
>
>





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