PCR of supercoils

Roland Hübner roland.hubner at ua.ac.be
Thu Jul 26 11:18:28 EST 2001


> I would greatly appreciate any observations or suggestions on how to 
> obtain the most product from PCR of supercoiled plasmids.    Any side-by-side
> comparisons of enzymes or conditions would be most helpful.  Thanks.

Hello Jim,

 well, in order to maximize PCR yield it is common to RE digest or 
topoisomerase treat dsDNA ccc molecules...  Several papers should be 
around in Biotechniques, PNAS, NAR, ... Focus  ;^)

 Are you tinkering up an optimized "yield polymerase mix"?

Best wishes,
 Roland




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