Bisulfite Treatment

Kelvin Joe kelvin121 at
Fri Jul 27 06:09:40 EST 2001

I try to investigate the methylation pattern of cancer. When I did bisulfite
treatment, I couldn't get enough DNA to do downsteam PCR. The problem might
be DNA degrade when incubated with sodium bisulfite.
Does anybody have the experience of bisulfite treatment? If you do, please
help me.

Thanks a lot.



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