SDS extraction of whole cells

Louis Hom lhom at OCF.Berkeley.EDU
Fri Jul 27 15:49:38 EST 2001


I would try using a sonicator to shear the DNA.  I've sonicated volumes as 
small as 50-100 ul using a tip sonicator in order to reduce the viscosity 
of SDS-PAGE samples.
-- 
______________________________________________________________________________
Lou Hom >K'93			     
lhom at ocf.berkeley.edu		
http://www.ocf.berkeley.edu/~lhom/ 	    




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