SDS extraction of whole cells

Warren Gallin wgallin at gpu.srv.ualberta.ca
Fri Jul 27 15:35:57 EST 2001


Hi folks,
	I am trying to do a Western blot on a set of cell lines and am trying
to keep the protein concentration as high as possible.  The problem is
that the DNA released on boiling in SDS makes the sample impossible to
pipette accurately.
	Aside from dilution, does anyone have a way to get the viscosity of
this kind of extract down to a workable level?

Thanks.

Warren Gallin




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