Bisulfite Treatment

Tina Bianco tina.bianco at adelaide.edu.au
Sun Jul 29 20:07:24 EST 2001


Hi Kelvin. I do a far bit of bisulfite treatment followed by PCR. Typically
I would bisulfite 1-2ug of DNA of which I would be able to do 8-10 PCRs from
(PCR product 500bp or less). The DNA is degraded (see Grunau et al Nucleic
Acids Res. 2001 Jul 1;29(13):E65-5.). For the technique I use see my
publication Hum Mutat. 1999;14(4):289-93.
I hope this helps Tina.


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