clean hamilton 0.2mm syringae
felipe.wettstein at ioez.tu-freiberg.de
Mon Jul 30 06:17:24 EST 2001
yes, it works, we could not rinse the syringes with a yellow pipet tip
but with a tube around the end of the syringe. it is a lot of work, but
finally some old syringaes work again. thank you very much
David L Haviland wrote:
> For cleaning Hamilton syringes I always used a combination of 4
> solutions. 1) DDH20, 2) 2% SDS, 3) 70% EtOH, and 4) Acetone.
> Go from 1 to 2, back to 1, then to 3, and finally 4, pulling about 10
> mls through each time. Use a 200ul pipette tip that is cut off that
> leads to an aspirator and stick the narrow end in the back of the
> Hamilton. Clean the plunger by dipping in the four solutions and
> wiping down each time.
> As a result, I've never lost a Hamilton this way and have been able
> to "re-claim" a few from the destiny of the trash can!!
> Hope this helps,
> David L. Haviland, Ph.D., Asst. Prof. Immunology
> University of Texas - Houston, H.S.C.
> Institute of Molecular Medicine, R907
> 2121 W. Holcombe Blvd., Houston, TX 77030
> If everything seems to be going so well, you have obviously
> overlooked something.
> ----- Original Message -----
> From: felipe wettstein <felipe.wettstein at ioez.tu-freiberg.de>
> Date: Friday, July 20, 2001 10:18 am
> Subject: Re: clean hamilton 0.2mm syringae
> > > > > may be taking place if the rinsing buffer is too cold: use
> > warm buffer?
> > > > we rinse with double destilled water, i assume it is at about room
> > > > temperature.
> > >
> > > ...have you tried rinsing in running buffer? Ie just dip the
> > needle in
> > > the gel buffer tank and rinse - presumably the sample buffer
> > does not
> > > precipitate in running buffer otherwise you would notice an artefact
> > > during a run
> > and you have no problems with blocked syringaes? to rinse in running
> > buffer is the first thought, i usually did before, but then
> > suddenly i
> > changed to water, .. hm... i will try.
> > thank you
> > felipe
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