Pseudomonas and pH

Michael Witty mw132 at mole.bio.cam.ac.uk
Mon Jul 30 08:18:04 EST 2001


Dear All,
        my aim is to pull down Pseudomonas aeruginosa using an antibody to
an outer membrane protein and protein A Sepharose beads.  Then I want to
disrupt the attachement to the beads and plate the cells on LB plates.  I
know that glycine buffer pH3.0 will separate IgG and protein A, BUT will
Pseudomonas aeruginosa survive this?

Is this a good or a dumb idea?  Regards, Mike.




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