SDS extraction of whole cells

Frank O. Fackelmayer Frank at Fackelmayer.de
Mon Jul 30 10:04:39 EST 2001



Michael Witty wrote:
> 
> Dear Frank,
>           is this for bacterial cells or mammalian cells?  Regards Mike.
> 


It actually works with both. Of course the cells must be disrupted by
SDS; I haven´t tried with gram positive bacteria, yeast or plant
material... The number of cells that can be processed is for HeLa cells.
The method can easily be scaled up when needed.

Frank




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