Michael Witty mw132 at
Fri Jun 1 05:21:46 EST 2001

On Thu, 31 May 2001, L R wrote:

> Hello,
> I am getting a lot of unspecific Ab binding doing a western.  If I am
> to use serum in the blocking buffer with the Ab, would it be the serum
> of the organism the Ab was raised in or the opposite serum (ie. for
> the primary Ab solution, the serum of the organism that the secondary
> Ab was raised in and vice-versa)?
> I greatly appreciate the help,
> LR
> lrzepiel at

Could you be adding to much sample to your gel?  Mike.

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