IMMUNOBLOTTING - NEED HELP
mw132 at mole.bio.cam.ac.uk
Fri Jun 1 05:21:46 EST 2001
On Thu, 31 May 2001, L R wrote:
> I am getting a lot of unspecific Ab binding doing a western. If I am
> to use serum in the blocking buffer with the Ab, would it be the serum
> of the organism the Ab was raised in or the opposite serum (ie. for
> the primary Ab solution, the serum of the organism that the secondary
> Ab was raised in and vice-versa)?
> I greatly appreciate the help,
> lrzepiel at uoguelph.ca
Could you be adding to much sample to your gel? Mike.
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