IMMUNOBLOTTING - NEED HELP

Michael Witty mw132 at mole.bio.cam.ac.uk
Fri Jun 1 05:21:46 EST 2001


On Thu, 31 May 2001, L R wrote:

> Hello,
>
> I am getting a lot of unspecific Ab binding doing a western.  If I am
> to use serum in the blocking buffer with the Ab, would it be the serum
> of the organism the Ab was raised in or the opposite serum (ie. for
> the primary Ab solution, the serum of the organism that the secondary
> Ab was raised in and vice-versa)?
>
> I greatly appreciate the help,
>
> LR
> lrzepiel at uoguelph.ca

Could you be adding to much sample to your gel?  Mike.




More information about the Methods mailing list