mnwoznia at med.uni-tuebingen.de
Fri Jun 1 15:50:03 EST 2001
Thanx a lot Wolfgang,
despite that coated coverslips is a good idea (I'm going to try it next
week) I had a look in some papers, and treated the cells with H89 in HEPES
buffered medium. Afterwards they seem to stay longer on the coverslips (I
have no idea why - any tips?).
I'll see what comes out next week if I coat them...:)
In Tübingen läuft wie immer, ich hoffe Dir geht's genauso gut:)
On Fri, 1 Jun 2001, Wolfgang Schechinger wrote:
> Dear Marcin,
> When you remove H89, will the cells re-attach again?
> Are you working with coated coverslips or are they pure glass?
> If you should need collagen or something, Kathrin and Cora in
> B2 should have some solution and protocols (and even the
> vacuum exsiccator that you'll need for the procedure).
> How do things go in Tuebingen?
> Viele Gruesse aus TaiPei,
> From: mnwoznia at med.uni-tuebingen.de (Marcin)
> Subject: H89 inhibitor
> Date sent: 31 May 2001 10:58:19 +0100
> Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre
> Originally to: methods at net.bio.net
> To: methods at hgmp.mrc.ac.uk
> > Dear all,
> > I'm trying to use H89 - a PKA specific inhibitor on transfected and
> > untransfected NIH3T3 cells. I added 5ul of 10mM DMSO solution to 1ml of
> > medium to reach the final concentration of 50uM. Unfortunately almost all
> > the cells swim away after 30 min, and I'm not able to fix them on glass
> > coverslips.
> > Do you have any hints?
> > I would greatly appreciate any help,
> > Marcin
> > ---
> [tiss mezzage wahs broduceRd using TYPO GENERATOR zoffwer]
> Dr. Wolfgang Schechinger
> Lab N233 (c/o Dr. Steve Roffler)
> Institute of Biomedical Sciences
> Academia Sinica
> 128 Yen-Chio Yuan Rd. Sec.2
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