H89 inhibitor

[Marcin]

Thanx a lot Wolfgang,
despite that coated coverslips is a good idea (I'm going to try it next
week) I had a look in some papers, and treated the cells with H89 in HEPES
buffered medium. Afterwards they seem to stay longer on the coverslips (I 
have no idea why - any tips?).
I'll see what comes out next week if I coat them...:)

In Tübingen läuft wie immer, ich hoffe Dir geht's genauso gut:)
Grüße,
Marcin

On Fri, 1 Jun 2001, Wolfgang Schechinger wrote:

> Dear Marcin,
> 
> When you remove H89, will the cells re-attach again?
> Are you working with coated coverslips or are they pure glass? 
> If you should need collagen or something, Kathrin and Cora in 
> B2 should have some solution and protocols (and even the 
> vacuum exsiccator that you'll need for the procedure).
> 
> How do things go in Tuebingen?
> 
> Viele Gruesse aus TaiPei, 
> 
> Wolfgang
> 
> 
> 
> From:           	mnwoznia at med.uni-tuebingen.de (Marcin)
> Subject:        	H89 inhibitor
> Date sent:      	31 May 2001 10:58:19 +0100
> Organization:   	BIOSCI/MRC Human Genome Mapping Project Resource Centre
> Originally to:  	methods at net.bio.net
> To:             	methods at hgmp.mrc.ac.uk
> 
> > Dear all,
> > I'm trying to use H89 - a PKA specific inhibitor on transfected and
> > untransfected NIH3T3 cells. I added 5ul of 10mM DMSO solution to 1ml of
> > medium to reach the final concentration of 50uM. Unfortunately almost all
> > the cells  swim away after 30 min, and I'm not able to fix them on glass
> > coverslips.
> > Do you have any hints?
> > I would greatly appreciate any help,
> > Marcin
> > 
> > 
> > ---
> > 
> > 
> 
> 
> [tiss mezzage wahs broduceRd using TYPO GENERATOR zoffwer]
> -----
> Dr. Wolfgang Schechinger
> Lab N233 (c/o Dr. Steve Roffler)
> Institute of Biomedical Sciences
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