genomic library screening by DNA binding protein

chopstixn chopstixn at
Sat Jun 2 15:13:24 EST 2001

With CHIP assay, you cannot screen the gene.  What I remember is that when
people cloned a DNA binding protein they first tried to define the target
Make oligo (random, 10bp approx.) flanked by primer site with restriction site,
perform gel shift by using synthetic oligo as a probe, purify the DNA from
shifted band, perform PCR with primers flanking the target oligo, repeat gel
shift, and subclone the purified oligo for sequencing.  

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