GPI anchor - how strong?

Phil phil at moonhead.trash.org.uk
Tue Jun 5 08:05:59 EST 2001


I would be interested to hear peoples views on the strength of a GPI
linkage.
I have expressed a protein on the surface of mammalian cells  - approx 500
EE 3
copies. Attempting to grab the cells with a corresponding mAb attached to a
paramagnetic bead I was surprised to see that the cells were not rosetted at
a frequency any higher than untransfected cells.
I assumed that the ligand, detecable by FACS using the same mAb, was being
yanked off the surface.
Though other explanations are possible.

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