rna from woody plant tissues

Michael L. Sullivan mlsulliv at facstaff.wisc.edu
Wed Jun 6 11:55:26 EST 2001


Does anybody have reccomendations for preparing RNA from woody plant
tissues.  I'd be especially interested in anyone who's successfully
modified a triazol protocol.  I've been able to get RNA, but at quite low
yields (e.g. 30 ug per gram of tissue).

One thought is that I just really need to use a lot of tissue, because that
actual amount of "cell contents" is relatively small compared to the amount
of "junk".

Thanks for any input.

Mike

Michael L. Sullivan, Ph.D

U.S. Dairy Forage Research Center
1925 Linden Drive West
Madison WI, 53706

(608) 264-5144 Phone
(608) 264-5147 Fax

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