In situ oligonucleotide probes

Thierry Giffon tgiffon at stanford.edu
Wed Jun 6 15:58:29 EST 2001


Dear Netters,

I'm starting to design some in situ hybridization experiments, (on
cyto-spinned cells), and I want to try using oligonucloetides labelled
with biotin in 5' as probes to detect some RNA messenger. Is there any
guidelines concerning the length and the Tm of the oligos, and how many
should I use in a cocktail ????

Thanks a lot for your help
Thierry




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