Glycerol stock longevity
Richard P. Grant
rpg14 at yahoo.co.uk.invalid
Thu Jun 7 10:03:47 EST 2001
In article <F17xcbz37cfJiY9ZNMj0001c18f at hotmail.com>,
bam_work at hotmail.com ("Betsy Moran") wrote:
> Does any one have information (data or opinion or personal experience) on
> long a bacterial glycerol stock is viable @ -80oC? How often to stocks
Opinion and experience - glycerol stocks remain viable 'for ever'.
*However* there is a risk of plasmid rearrangement, even at -80, as I
have experienced - for stocks that were 3 years old or less.
Store plasmids as DNA in water or TE: mid-term at 4C in TE; for longer
term/stock storage freeze at -20 (water or TE).
 'for ever' = as long as mol biol has been a science ;)
Richard P. Grant 0x5F9559B1 MRC Lab of Mol Biol
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