Glycerol stock longevity

[Richard P. Grant]

In article <F17xcbz37cfJiY9ZNMj0001c18f at hotmail.com>, 
bam_work at hotmail.com ("Betsy Moran") wrote:

> Does any one have information (data or opinion or personal experience) on 
> how
> long a bacterial glycerol stock is viable @ -80oC?  How often to stocks 
> fail 
> to
> regrow? 


Opinion and experience - glycerol stocks remain viable 'for ever'[0].  
*However* there is a risk of plasmid rearrangement, even at -80, as I 
have experienced - for stocks that were 3 years old or less. 


Store plasmids as DNA in water or TE: mid-term at 4C in TE; for longer 
term/stock storage freeze at -20 (water or TE).


HTH

r


[0] 'for ever' = as long as mol biol has been a science ;)

-- 
Richard P. Grant  0x5F9559B1                      MRC Lab of Mol Biol


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