Glycerol stock longevity

Richard P. Grant rpg14 at
Thu Jun 7 10:03:47 EST 2001

In article <F17xcbz37cfJiY9ZNMj0001c18f at>, 
bam_work at ("Betsy Moran") wrote:

> Does any one have information (data or opinion or personal experience) on 
> how
> long a bacterial glycerol stock is viable @ -80oC?  How often to stocks 
> fail 
> to
> regrow? 

Opinion and experience - glycerol stocks remain viable 'for ever'[0].  
*However* there is a risk of plasmid rearrangement, even at -80, as I 
have experienced - for stocks that were 3 years old or less. 

Store plasmids as DNA in water or TE: mid-term at 4C in TE; for longer 
term/stock storage freeze at -20 (water or TE).



[0] 'for ever' = as long as mol biol has been a science ;)

Richard P. Grant  0x5F9559B1                      MRC Lab of Mol Biol

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