Glycerol stock longevity

David L Haviland David.L.Haviland at uth.tmc.edu
Thu Jun 7 10:33:08 EST 2001


Besty:

I have not found that necessary for bacterial stocks, particularly so after
just thawing one out dated 10/10/95.

However, for tissue culture cells I find it in my best interest to have
anything older than 4 years thawed out grown for a week and refrozen.  The
extra effort definately helps in the recovery time when I really need the
cells.

Hope this helps,
David

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