protein quantification

Patrick Lynch
Thu Jun 7 10:33:03 EST 2001


I use Biorad's protein assay dye.  All standards (from 62.5 ug/ml - 500
ug/ml BSA) and sample volumes are set at 10 ul and pipetted into a 96 well
plate.  200ul of dye (diluted 1/5 with H2O) are added to each well using a
multichannel pipetteman, wait 5 min and read at OD600.

Hope this helps,

Patrick



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