E. coli preservation

R. Jayakumar jakku at mrna.tn.nic.in
Tue Jun 12 05:40:09 EST 2001


Dear Kami
     I am in India where the powersupply is much worser than yours.  Here
the latest light emitting device is the candle, since the batteries in our
flashlight often gives out, considering the powercuts often goes to 4-5
hours or more and we are in one of the best states in the country.  :-)))
But we store our E.coli cultures at -70C.  The cultures often withstand
freeze and thaw against all accepted theories.  Must be God's blessing..
:-)))  I don't see any other better way of storing E.coli cultures for years
than this.  Another way you can do it is by storing it as lyophilized
cultures, which can be stored in the refrigerator or at room temperature in
vacuum sealed glass ampules using a lyophilizer and a glass sealing
equipment.
   best of luck
jayakumar

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R. Jayakumar
Dept. of Molecular Microbiology,
School of Biotechnology,
Madurai Kamaraj University,
Madurai - 625021. India.
tel: +91-452-859141(night hours), +91-452-858471 (ask to connect to 374)
fax:+91-452-859105
Efax: (603)-688-4665
Email: jakkku at mrna.tn.nic.in, (send a copy to jakku at zxmail.com ....just in
case)
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Science is constructed of facts like a house is of bricks, but a mere
accumulation of facts does not make science nor does a pile of bricks a
house
-Henri Poincare
----- Original Message -----
From: "Jim Kami" <jakami at ucdavis.edu>
To: <methods at hgmp.mrc.ac.uk>
Sent: Tuesday, June 12, 2001 1:17 AM
Subject: E. coli preservation


> Hi,
>     Does anyone know of a good method for preserving E. coli cells for
> long periods (several years) at room temperature ? We are currently
> developing several BAC libraries and storing the primary plates at -70C,
> however, we are in California and can not be certain of  a continuous
> electrical supply. (note: the latest, most popular, handheld, electronic
> device sweeping the state is the flashlight) Any ideas would be greatly
> appreciated.
>
> Thanks
>
> Jim Kami
> Dept of Agronomy
> University of California
> Davis, California  95616
>
>

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