Protein extraction question.

Dima Klenchin klenchin at REMOVE_TO_REPLY.facstaff.wisc.edu
Tue Jun 12 07:58:21 EST 2001


jul2 at pitt.edu wrote:

:I have my proteins in the lysis buffer which contains triton.
:If I need to extract my proteins using TCA procedure, will
:the triton go with the proteins?

Isn't this a two minutes experiment (triton solution + TCA)? 
I did it long ago and AFAIR a white precipitate forms.

        - Dima




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